Lindsey Boylan tweeted that the Democratic governor "sexually harassed me for years. Many saw it, and watched."
"I could never anticipate what to expect: would I be grilled on my work (which was very good) or harassed about my looks. Or would it be both in the same conversation? This was the way for years," she continued.
Asked for comment, Cuomo's press secretary Caitlin Girouard said, "There is simply no truth to these claims."
Boylan, 36, worked for the Cuomo administration from March 2015 to October 2018, serving first as executive vice president of Empire State Development and then as a special adviser to Cuomo for economic development.
She did not provide details of the alleged harassment and didn't immediately respond to messages from The Associated Press. Boylan later tweeted, "To be clear: I have no interest in talking to journalists. I am about validating the experience of countless women and making sure abuse stops."
Boylan's sexual harassment allegation against Governor Andrew M. Cuomo comes after The Associated Press and others reported that the 63-year-old governor is under consideration for the job of attorney general in the administration of President-elect Joe Biden.
Earlier this month, Boylan had also tweeted about her work experience in the Cuomo administration, listing it as the worst job she ever had.
"I tried to quit three times before it stuck. I've worked hard my whole life. Hustled — fake it till you make it style," she wrote. "That environment is beyond toxic. I'm still unwrapping it years later in therapy!"
Personnel memos written in 2018, obtained by The Associated Press, indicate that Boylan resigned after she was confronted about complaints about her own office behavior.
Several women complained to Empire State Development's human resources department that "Ms. Boyland had behaved in a way towards them that was harassing, belittling, and had yelled and been generally unprofessional," wrote the administration's ethics officer, Julia Pinover Kupiec in one memo.